Acid-fast stain acid-fast microorganisms have mycolic acids present in their cell walls which assists with attaching a stain into these cell that will be resistant to decolorization there are two different kinds of acid-fast staining procedures: the ziehl-neelsen (zn) method and the kinyoun (k) method. Pancreatic tuberculosis (tb) is a relatively rare disease that can mimic carcinoma, lymphoma, cystic neoplasia, retroperitoneal tumors, pancreatitis or pseudocysts. The acid-fast stain uses three different reagents: carbol fuchsin, acid-alcohol, and methylene blue carbol fuchsin is a dark red stain that is soluble in the lipid substances foundin the cell wall of the mycobacteral cell.
Bacterial classification, structure and function introduction the purpose of this lecture is to introduce you to terminology used in microbiology the lecture will: (kinyoun or acid fast stain) are therefore used that take advantage of the resistance to destaining after lengthier initial staining. Acidfast stain: background and introduction mycobacterium and many nocardia species are called acid-fast because during an acid-fast staining procedure they retain the primary dye carbol fuchsin despite decolorization with the powerful solvent acid-alcoholnearly all other genera of bacteria are nonacid-fastthe acid-fast genera have lipoidal mycolic acid in their cell walls. Acid‐fastness is an uncommon characteristic shared by the genera mycobacterium (section 10a) and nocardia because of this feature, this stain is extremely helpful in identification of these bacteria.
Acid-fastness is a physical property of certain bacterial and eukaryotic cells, as well as some sub-cellular structures, specifically their resistance to decolorization by acids during laboratory staining procedures once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name acid-fast. Decorative concrete is the use of concrete as not simply a utilitarian medium for construction but as an aesthetic enhancement to a structure, acid staining, decorative overlays, polished concrete, concrete countertops, vertical overlays and more. This method of staining uses the gram stain and the acid-fast stain gram staining is the most useful, as it will classify bacteria into either a gram positive or gram negative group this is carried out by applying a heat-fixed smear with a primary stain, (usually a purple dye. Home free essays lab report about simple staining of microbes lab report about simple staining of microbes essay for negative staining, the negative stain uses the dye nigrosin, which is an acidic dye by giving up a proton (as an acid) the chromophore of the dye becomes negatively charged we will write a custom essay sample on lab.
The counter stain is used to colorize the non-acid fast cells present in the culture the cells do not have the type of cell wall that requires heat application for the stain to penetrate a child presents symptoms suggestive of tuberculosis, namely a respiratory infection with a productive cough. The introduction to lab unit 05 must be written in essay form, and contain background information on the acid-fast stain, endospore stain, and fluid thioglycollate media the following are specific topics to include. The acid-fast stain robert koch was the first person to isolate and identify mycobacterium tuberculosis from a patient with tuberculosis he developed a stain for the bacterium, although it was. The smear is cover with carbonaceous for 5 minutes then was the slide carefully with distilled water 2 when washing the slide you should tilt the slide and aim the water above the smear washing over it 3.
Acid-fast mycobacteria contain mycolic acid in their outer membrane, making the cells waxy and resistant to staining with aqueous based stains such as the gram stain the primary stain, carbolfuchsin, is applied to the cells and phenol is used to allow the stain to penetrate into the waxy surface of acid-fast microorganisms. The acid-fast staining technique consists of using carbol fuschin, a lipid-soluble dye which is them steamed to allow penetration of the mycobacterial cell wall the slide is then washed with an acid-alcohol destain and stained again using methylene blue. Acid fast vs non acid fast bacteria the difference between acid fast and non acid fast bacteria is in their cell wall, basically bacteria are generally identified and observed with differential staining procedures acid fast staining is one of such methods to distinguish a certain type of bacteria from others. Acid fast stains are used to differentiate acid fast organisms such mycobacteria acid fast bacteria have a high content of mycolic acids in their cell walls acid fast bacteria will be red, while nonacid fast bacteria will stain blue/green with the counterstain with the kinyoun stain.
A method of staining used in bacteriology in which a smear on a slide is treated with carbol-fuchsin stain or auramine-rhodamine stain, decolorized with acid alcohol, and counterstained with methylene blue or potassium permanganate to identify acid-fast bacteria. Excerpt from essay : microorganisms through a microscope the gram stain test distinguishes two fundamental cell varieties of bacteriathe latter also determines the presence of bacteria gram-positive refers to the type that retains the original crystal violet stain. The acid-fast organisms are high in myolic acid and the gram stain is not able to work properly because of the high acidity 13 list two acid-fast organisms and the respective diseases they cause.
During the acid-fast staining technique, a slide-shaped piece of blotting paper is placed over the bacterial smear, a bright pink dye (ziehl’s carbolfuchsin) is then applied to the blotting paper, and the slide and paper are placed over a water bath (a steaming pot of water covered with a screen) for 3 – 5 minutes. The gram stain/acid fast staining practice the endospore stain practice begins chapter test: 100 multiple choice + 2 essays over ch 3 + safety, smears, stains, the microscope care, focusing, cleaning, & storage. Stains and dyes for microbiology thermo scientific™ remel™ gram stain kit, 4 x 250 ml bottles thermo scientific™ remel gram stain kit is for qualitative procedures to differentiate gram-positive from gram-negative microorganisms. A standardized, highly reproducible modification of the original alfert & geschwind procedure (1953) has been formulated it involves trichloroacetic acid extraction at 60°c and the use of a weak hcl-borate buffer for the dye solution a comparison of sections stained with fast green, feulgen.